Skeletal muscle activation induces a slow calcium signal which is associated with gene expression. It has been shown that the release of ATP from cells participates in the signal transduction pathway that induces this response, but the specific receptors involved are unknown. The aim of this study was to gain a first insight into the receptors involved in this gene expression signaling pathway. Rat skeletal miotubes were incubated with specific receptors agonists (ATP, ADP, UTP, UDP and adenosine) at different concentrations. Thereafter, interleukin-6 (IL-6) and c-fos mRNAs were analyzed through the RT-PCR method, to determine the gene expression response to different extracellular receptors activation. High ATP (activates all purinergic receptors) concentrations induced increases in the gene expression of both IL-6 and c-fos, while high ADP (P2Y1 receptor) concentrations only induced increases in IL-6 mRNA. Neither UTP (receptors P2Y2 and P2Y4) nor UDP (receptor P2Y6) induced gene expression. Stimulation with adenosine (specific adenosine receptors) instead, showed a time and concentration dependent gene expression response, increasing mRNA for both IL-6 and c-fos but under different conditions. Thus, the results show that P2Y1 and adenosine receptors activation leads to gene expression, suggesting their participation in the slow calcium signaling pathway.
Key words: slow calcium signal, nucleotide receptors, interleukin-6, c-fos
Hi Rodrigo,
ResponderEliminarWell done. Your abstract reads very well. Here go some suggestions to improve it before Thursday midnight.
1. In the methods section, instead of using through the RT-PCR method, use with or by means of and delete the comma.
2. In the results section, the subject of the sentence is ACTIVATION, so you need to clarify what their refers to.
Good job,
See you on Friday,
Claudia