Extracellular ATP, ADP and adenosine stimulation induces gene expression in skeletal miotubes

Skeletal muscle activation induces a slow calcium transient which is associated with gene expression. There has been shown that the release of ATP from cells participates in the signal transduction pathway that induces this response, but the specific receptors involved are unknown. The aim of this study was to gain a first insight into the receptors involved in this gene expression signaling pathway. Rat skeletal miotubes were incubated with specific receptors agonists (ATP, ADP, UTP, UDP and adenosine) at different concentrations. Thereafter, interleukin-6 (IL-6) and c-fos mRNAs were analyzed through the RT-PCR method, to determine the gene expression response to different extracellular receptors activation. High ATP (activates all purinergic receptors) concentrations induced increases in the gene expression of both IL-6 and c-fos, while high ADP (P2Y1 receptor) concentrations only induced increases in IL-6 mRNA. Neither UTP (receptors P2Y2 and P2Y4) nor UDP (receptor P2Y6) induced gene expression. Stimulation with adenosine (specific adenosine receptors) instead, showed a time and concentration gene expression response, increasing mRNA for both IL-6 and c-fos but under different conditions. Thus, the results show that P2Y1 and adenosine receptors activation leads to gene expression, suggesting their participation in the slow calcium signaling pathway.

Key words: slow calcium signal, nucleotide receptors, interleukin-6, c-fos

PRESENTATION

My name is Rodrigo Fernández, I am a Physical Trainer graduated at the University of Santiago of Chile. I am at the second year of the Physiology Master at the University of Santiago of Chile. I am interested in the research of skeletal muscle adaptations in response to different types of exercise.

In the future, I would like to study abroad something related specifically to exercise physiology, so this is the main reason why I want to learn English.

I hope this course helps me to be able to speak (or try to speak) English in front to other people.

Presentation

My name is Rodrigo, i'm a physical trainer graduated at the University of Santiago of Chile. I'm at the second year of the Master in Physiology at the University of Chile. I'm interested in the research of the skeletal muscle adaptations in response to different types of exercise.

In the future, i would like to study something related to exercise physiology in other country, so this is the main reason why i want to learn english.

I hope this course helps me to be able to speak (or try to speak) in english in front of other people.